Figure 3

smRNA-seq reveals novel miRNAs in endothelial lineage cells. (A) A flowchart describes the data analysis pipeline. The numbers of sequences and reads remaining at each step of the data analysis are indicated. Reads numbers include sequences map to more than one locus in the genome. (B) Deduced RNA secondary structures of a set of newly discovered miRNAs. (C) A Venn diagram showing unique and common novel miRNAs in each cell type. (D) The top ten differentially expressed novel miRNAs between late EPC and other cell types. Areas and numbers above the circles indicate miRNA RPM values in late EPCs. (E) Validation of smRNA-seq data by RT-qPCR. All histograms were graphed as mean ± standard deviation (n = 3) **: p < 0.01, ***: p < 0.001.