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Table 1 Bacterial strains and plasmids used in this study

From: Unlocking the mystery of the hard-to-sequence phage genome: PaP1 methylome and bacterial immunity

Strains or plasmids Relevant characteristicsa Source or reference
Strains   
Pseudomonas aeruginosa PA1 Belongs to serum typing group 9 of P. aeruginosa international antigenic typing system; host of phage PaP1 Laboratory collection
P. aeruginosa PA3 Belongs to serum typing group 6 of P. aeruginosa international antigenic typing system; host of phage PaP3 Laboratory collection
E. coli DH5α Host for the construction of shotgun library clones Promega, WI, USA
E. coli DH5α cat+nfi The nfi gene is replaced with cat. This study
E. coli DH5α Δnfi The nfi gene is knocked out. This study
Plasmids   
pKD3 Template plasmid for Red system; Ampr, Cmr [26]
pKD46 Red expression plasmid; Ampr [26]
pCP20 Flp expression plasmid; Ampr, Cmr [25]
pUC18 Vector for the construction of shotgun library clones; Ampr TaKaRa, Shiga, Japan
  1. aAmpr, ampicillin resistant; Cmr, chloramphenicol resistant.