Table 1 Bacterial strains and plasmids used in this study

Strains

Pseudomonas aeruginosa PA1

Belongs to serum typing group 9 of P. aeruginosa international antigenic typing system; host of phage PaP1

Laboratory collection

P. aeruginosa PA3

Belongs to serum typing group 6 of P. aeruginosa international antigenic typing system; host of phage PaP3

Laboratory collection

E. coli DH5α

Host for the construction of shotgun library clones

Promega, WI, USA

E. coli DH5α cat⁺:Δnfi

The nfi gene is replaced with cat.

This study

E. coli DH5α Δnfi

The nfi gene is knocked out.

This study

Plasmids

pKD3

Template plasmid for Red system; Amp^r, Cm^r

[26]

pKD46

Red expression plasmid; Amp^r

[26]

pCP20

Flp expression plasmid; Amp^r, Cm^r

[25]

pUC18

Vector for the construction of shotgun library clones; Amp^r

TaKaRa, Shiga, Japan