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Table 2 Primers and other DNA sequences used in this study

From: Unlocking the mystery of the hard-to-sequence phage genome: PaP1 methylome and bacterial immunity

Primers or other DNA sequencesa

Sequence (5′-3′)

Target genes or locations

Construction of the nfi mutant

Cm-F

GTGTAGGCTGGAGCTGCTTC

Chloromycetin-resistant gene of pKD3

Cm-R

CATATGAATATCCTCCTTAG

 

Nfi-F

TGTGCCGCCAGAACATGC

nfi gene of E. coli DH5α

Nfi-R

GACGCAGATGAATTGGGT

 

H1

CGTGGAGGCAGTGCATCGACTGTCTGAACAGTATCACCGCTAAGGAGTGATTATG

Upstream of the nfi gene

H2

TTTGTAACATGTTGAGTTCTCAAATACGGAAATTATCCGCAGTTTACCTGAATTA

Downstream of the nfi gene

  1. aPrimers and other DNA sequences were synthesised by BGI-Shenzhen (Shenzhen, China).
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BMC Genomics

ISSN: 1471-2164

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