De novo transcriptome assembly for rudimentary leaves in Litchi chinesis Sonn. and identification of differentially expressed genes in response to reactive oxygen species

Table 2 Throughput and quality of RNA-seq of the reference library and the DGE libraries

Libaries	Total	Total Nucleotides	Q20 percentage	N percentage	GC percentage
Libaries	Reads	(nt)	Q20 percentage	N percentage	GC percentage
Reference library	61,338,190	6,133,819,000	97.82%	0.01%	46.73%
Lc0h-1	30,382,747	1,488,754,603	98.51%	0.01%	46.11%
Lc0h-2	30,181,112	1,478,874,488	98.53%	0.01%	45.85%
Lc5h-1	28,942,560	1,418,185,440	98. 45%	0.01%	46.11%
Lc5h-2	32,276,093	1,581,528,557	98.48%	0.01%	46.28%
Lc10h-1	30,733,719	1,505,952,231	98.50%	0.01%	45.90%
Lc10h-2	31,382,314	1,537,733,386	98.51%	0.01%	45.83%

One reference library was constructed by mixing RNA extracted from ROS-treated rudimentary leaves in 0 h, 5 h and 10 h of treatment. 6 DGE libraries were constructed from 0 h, 5 h and 10 h of ROS-treated rudimentary leaves. Each time point of treatment had 2 biological replicates. All libraries were sequenced using HiSeq 2000. Q20 percentage indicates the percentage of sequences with sequencing error rate lower than 1%. N percentage is the percentage of nucleotides which could not be sequenced.

ISSN: 1471-2164