Figure 1

Schematic of T. gondii /host dataset generation. (A) Nine mice were divided into three experimental groups: uninfected, 10 days post-infection, and 28 days post-infection. Infected mice were given 10,000 type II ME49 parasites and sacrificed on the corresponding days. The forebrains were removed and homogenized in TRIzol, and RNA was extracted and purified. A cDNA library was generated from the RNA prior to IlluminaHiSeq2000 sequencing. Raw reads were aligned to either the T. gondii or M. musculus genomes, normalized and analyzed for differential gene expression. (B) To examine T. gondii in the brains of mice at the designated time points, mice were infected with 10,000 parasites of a bioluminescent T. gondii[62]. Shown are representative brains for 10 and 28 day post-infection mice, after the brains were soaked for 5 minutes in luciferin prior to imaging.