Skip to main content
Figure 10 | BMC Genomics

Figure 10

From: High dose ionizing radiation regulates micro RNA and gene expression changes in human peripheral blood mononuclear cells

Figure 10

Validation of the miRNA data by qPCR. qPCR revealed a strong correlation between the microarray and PCR data. Expression values were normalized relative to values in non-irradiated PBMCs. The expression of miR-99b* (A), miR-887 (B), and miR-4299 (C), was significantly higher in irradiated PBMCs compared to non-irradiated PBMCs 20 hours after irradiation. Relative gene expression levels were calculated based on the mean value of four samples using the comparative Ct method. RNU44 served as internal reverence miRNA. Data are presented as the mean + SD. *p < 0.05, paired T-test; n = 4.

Back to article page