Figure 2
Proteins involved in executing apoptosis are up-regulated in response to ionizing radiation. A membrane array detecting 35 apoptosis-related proteins was incubated with cell lysates from 106 non-irradiated (A) or irradiated (B) human PBMCs 20 hours after exposure. (C) The legend for the spotted proteins. (D) The average pixel intensities of two independent experiments were averaged and are depicted as the fold change in up-regulation of irradiated PBMCs compared to non-irradiated PBMCs. For relative quantification in (C), an exposure time of 60 seconds was used for proteins with high expression values, and an exposure time of 625 seconds was used for proteins with lower expression values. Data are presented as mean + SD; n = 2.