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Figure 4 | BMC Genomics

Figure 4

From: High dose ionizing radiation regulates micro RNA and gene expression changes in human peripheral blood mononuclear cells

Figure 4

Principle component analysis (PCA) of mRNA expression data shows differences in response to irradiation and cultivation. Samples are displayed with respect to the first three components and are colored with respect to radiation and time point. A total of 28 unique experiments were performed and are displayed in the figure. Matched samples of irradiated and non-radiated PBMCs from four different donors at 2, 4, and 20 hours, as well as expression data from PBMCs isolated immediately after venipuncture without any cultivation period, were used for mRNA expression analysis. PCA allows visual identification of data patterns and highlights similarities and differences between samples. PCA was performed using GeneSpring and was based on conditions. All conditions can be clearly separated from each other. Irradiated PBMCs built three distinct clusters on the right side of the figure, displaying relative low X-axis values. In contrast, non-irradiated PBMCs formed three visible clusters, displaying completely distinct X-axis values compared to irradiated PBMCs, whereas the Y-axis and Z-axis values were comparable. The expression data for 0 h PBMCs formed a distinct cluster separate from 2 or 4 hours after cultivation, even without irradiation. 0 h = PBMCs without any cell culture period; rad = irradiated samples; vit = non-irradiated samples.

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