Figure 5

Ionizing radiation induces alterations in mRNA expression levels. (A) mRNA changes in irradiated human PBMCs incubated for 2, 4, and 20 hours using the Agilent Whole Human Genome Oligo Microarray (8 × 60 K) are shown. Venn diagram showing the overlap of up- and down-regulated genes with significant changes in expression 2, 4, and 20 hours after irradiation. A time-dependent increase in differentially expressed genes was observed. The greatest alterations in gene expression between irradiated and non-irradiated PBMCs were detected 20 hours after exposure. The inner triangle contains 31 transcripts that were differentially expressed in all samples at all time points. (B) Heat map showing the expression values of the 31 transcripts that were differentially expressed at all time points in all donors. The range of expression was from −2.3 (green, down-regulation) to 2.3 (red, up-regulation). Each time point can be clearly discriminated, and irradiated samples can be discriminated from non-irradiated samples. miRNA names are given on the right border of the heat map.