Figure 8
Regulatory network of hepatic leukemia factor (HLF) displaying the interplay among miRNAs, HLF, and mRNAs. (A) The network was constructed using MAGIA2, which incorporates positive and negative correlations between expression profiles of possibly interacting miRNAs, transcription factors, and genes according to their predictions. These miRNAs were up-regulated in irradiated cells 20 hours after exposure, whereas their target mRNAs were down-regulated. mRNAs, transcription factors, and miRNAs are shown as light blue circles, green circles, and orange triangles, respectively, and positive and negative correlations are shown as red and blue arrows, respectively. (B) qPCR of HLF shows a significant reduction in HLF expression 20 hours after IR exposure, n = 3. (C) Cells were harvested 20 hours after irradiation and the HLF level assessed by immunoblotting. IR significantly reduced the HLF protein level compared to non-irradiated samples.