Expression of the selected 12 transcription factors by qRT-PCR. Expression patterns of the selected 12 transcription factors in the wing disc at indicated six developmental stages, which are 3-day-old fifth instar larvae (L5D3), L5D6, 1-day-old wandering (W1), PP, P0 and 3-day-old pupae (P3). (A) Bm_nscaf2589_261 (probable nuclear hormone receptor HR38). (B) Bm_nscaf1898_501 (D-ETS-4-like isoform X2). (C) B m_nscaf2876_46 (bHLH protein 15-like). (D) Bm_nscaf2847_349 (protein escargot-like). (E) Bm_nscaf2888_249 (nuclear receptor GRF). (F) Bm_nscaf2902_040 (helix-loop-helix protein delilah-like). (G) Bm_nscaf3090_4 (chorion specific C/EBP). (H) Bm_scaffold316_2 (protein embryonic gonad-like). (I) Bm_nscaf1898_212 (ets DNA-binding protein pokkuri-like). (J) Bm_nscaf2770_58 (transcription factor SOX-4-like). (K) Bm_nscaf2847_110 (Krueppel-like factor 10-like). (L) Bm_nscaf2930_060 (enhancer of split mbeta). The relative expression levels were normalized to the Bmβ-actin levels. The values are mean ± SEM (n = 3). Different letters indicate statistical significance (p < 0.05).