Expression analysis of cuticular or cuticle protein coding genes. The RPKM values of undetected genes are viewed as 0.001. Cluster 3.0 software was used to plot the heat map (Similarity Metric: Euclidean distance, Clustering method: Complete linkage), and Treeview software was used to generate the heat map. The colors in the map display the relative values at the given 3 developmental stages. Green indicates the lowest level of expression, black indicates the intermediate level of expression, and red indicates the highest level of expression. (A) Distribution of cuticular or cuticle protein coding genes at the three stages. (B) Heat map of hierarchical clustering of 148 differentially expressed cuticle protein genes.