qRT-PCR results of expression of the chitin degradation related genes. The expression patterns of two chitin degradation genes in the wing disc at six developmental stages, which are 3-day-old fifth instar larvae (L5D3), L5D6, 1-day-old wandering (W1), PP, P0 and 3-day-old pupae (P3) The relative expression levels were normalized to the Bmβ-actin levels. (A) Bm_nscaf2993_229 (chitin deacetylase 4). (B) Bm_nscaf3031_201 (β-N-acetylglucosaminidase 1 precursor). The values are the mean ± SEM (n = 3) of three repeat experiments using qRT-PCR. Different letters indicate statistical significance (p < 0.05).