Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis

BMC Genomics

Table 8 Expression patterns of the genes related to wing disc early development

Gene	RNA-seq ID	L5D6-RPKM	PP-RPKM	P0-RPKM	L5D6 vs PP	PP vs P0
engrailed	Bm_nscaf2964_172	57.41	27.17	25.47	-1.08*	-
Hedgehog	Bm_nscaf3048_40	13.70	5.56	3.24	-	-
decapentaplegic	Bm_nscaf2993_221	38.81	22.75	17.10	-	-
apterous AE	Bm_nscaf2210_128	117.49	57.39	27.28	-1.03*	-
apterous AF	Bm_nscaf2210_130	31.10	27.52	8.94	-	-1.62*
apterous Bβ	Bm_nscaf2210_132	95.88	83.98	16.08	-	-2.38*
Fringe ¹	Bm_nscaf2860_94	13.82	40.86	15.17	1.56*	-1.43*
Fringe ¹	Bm_nscaf2860_96	45.94	74.37	28.61	+	-1.37*
delta	Bm_nscaf2986_021	12.75	6.39	3.16	-	-
serrate	Bm_nscaf1705_01	83.35	35.30	18.98	-1.24*	-
Notch ¹	Bm_nscaf2888_347	9.04	4.05	3.31	-	-
	Bm_nscaf2888_348	5.91	4.25	9.16	-	+
	Bm_nscaf2888_349	17.65	14.18	7.54	-	-
Wingless(precursor)	Bm_nscaf2847_167	17.20	5.51	0.86	-1.64*	-
vestigial	Bm_nscaf3056_42	17.08	7.54	7.70	-	+
scalloped	Bm_nscaf1898_116	52.42	25.22	14.10	-1.06*	-
Scalloped isoform 2	Bm_nscaf1898_117	44.05	35.26	8.31	-	-
achaete-scute 2	Bm_nscaf1898_167	2.04	7.54	40.06	+	2.41*
urbain	Bm_nscaf2902_362	45.40	11497.96	161.44	7.98*	-6.15*

¹These genes have more than one RNA-Seq sequences. The gene expression level was calculated by RPKM method. "+" represents up-regulation, and "-" represents down-regulation. Asterisks indicate that the gene, whose expression level had more than two folds difference between two stages and False Discovery Rate (FDR) was less than 0.001, was identified as differentially expressed at this developmental stage. And the values in the last two columns were calculated by Log₂(PP-RPKM/L5D6-RPKM) or Log₂(P0-RPKM/PP-RPKM) and only those differentially expressed genes are given.

ISSN: 1471-2164