Gene duplication and protein size box-plot for all sequences predicted to be secreted or non-secreted. Mean protein sizes, represented as black dots, were compared with a one-tailed Student’s t-test. *Secreted mean smaller than the non-secreted mean with same copy number, **Non-secreted mean greater than one copy less than the non-secreted mean, ***Secreted mean greater than one copy less than the secreted mean, ****Secreted mean smaller than one copy of the less secreted mean. All p-values < 0.001. Four or more copies were grouped together.