Figure 3

Expression patterns of SlCLE genes in selected tissues and fruits at different developmental stages. (A) Photos of tomato fruits at seven developmental stages assessed. (B) Transcription levels of SlCLEs investigated by RT-qPCR shown as heat-map for better illustration. Relative gene expression levels were normalized to have mean zero and variance one with actin transcript values before producing the heat maps. The RT-qPCR analyses were repeated at least three times in three independent experiments. Color scale at the top of each dendrogram represents log2 values. Total RNA isolated from apex (A), root (R), young, medium and old stems (YS, MS, and OS), young and mature leaf (YL and ML), flower (FL), flower buds (FB), and 1-cm, 2-cm and 3-cm sized green fruits (F1, F2 and F3), mature green fruit (MG), fruit at breaker stage (BF), fruit turning red (TF) and mature fruit in red(RF).