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Figure 4 | BMC Genomics

Figure 4

From: A universal protocol to generate consensus level genome sequences for foot-and-mouth disease virus and other positive-sense polyadenylated RNA viruses using the Illumina MiSeq

Figure 4

Genome coverage profiles for three non-FMDV panel of viruses. Coverage of 10,000 was achieved for the majority of the EMCV-1 genome (olive). Peaks in coverage can be observed at the location of sequence specific primers used in the RT reaction (~4000 bp and ~8000 bp). A dip in coverage was evident at the poly(C) tract. The ERAV-1 genome showed between 10x and 100x coverage with visible peaks in coverage at the specific primer sites (~4000 bp and ~8000 bp) (black). Approximately 100x coverage of the majority of the VESV-B34 genome was achieved (blue).

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