Methyltransferases acquired by lactococcal 936-type phage provide protection against restriction endonuclease activity

Table 3 Summary of putative MTases in the 936-type phage

Phage	Locus tag	Gene designation	Protein	Genome location	Target motif	Type	Group	Conserved motifs
Phi15	Phi15_36	mtPhi15-1	M.Phi15I	Replication	5′-CC^6mAG-3′^a	II	α	X, I, II, IV, VI, VIII
Phi93	Phi93_02	mtPhi93-1	M.Phi93I	Packaging	5′-GGWG^6mA-3′^b	II	β	IV, VI, X, I, II
	Phi93_04	mtPhi93-DAM	M.Phi93DAM	Packaging	5′-G^6mATC-3′^c	II	-	IV
	Phi93_39	mtPhi93-2	M.Phi93II	Replication	5′-CY^6mAG-3′^d	II	α	X, I, II, IV, VI, VIII
Phi145	Phi145_02	mtPhi145-1	M.Phi145I	Packaging	5′-GGWG^6mA-3′^b	II	β	IV, VI, X, I, II
	Phi145_37	mtPhi145-2	M.Phi145II	Replication	5′-CY^6mAG-3′^d	II	α	X, I, II, IV, VI, VIII

^aMotif identified on two independent SMRT sequencing runs with Phi15 propagated on two separate hosts.
^bValidated by restriction analysis using HphI. Phi93_002 and Phi93_039 are 99% and 100% identical to Phi145_002 and Phi145_037 respectively, therefore only the genes from Phi145 were cloned and validated by restriction analysis.
^cValidated by restriction analysis using DpnI and DpnII.
^dAssigned based on comparative sequence alignment to mtPhi15-1.

ISSN: 1471-2164