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Table 3 Summary of putative MTases in the 936-type phage

From: Methyltransferases acquired by lactococcal 936-type phage provide protection against restriction endonuclease activity

Phage Locus tag Gene designation Protein Genome location Target motif Type Group Conserved motifs
Phi15 Phi15_36 mtPhi15-1 M.Phi15I Replication 5′-CC6mAG-3′a II α X, I, II, IV, VI, VIII
Phi93 Phi93_02 mtPhi93-1 M.Phi93I Packaging 5′-GGWG6mA-3′b II β IV, VI, X, I, II
  Phi93_04 mtPhi93-DAM M.Phi93DAM Packaging 5′-G6mATC-3′c II - IV
  Phi93_39 mtPhi93-2 M.Phi93II Replication 5′-CY6mAG-3′d II α X, I, II, IV, VI, VIII
Phi145 Phi145_02 mtPhi145-1 M.Phi145I Packaging 5′-GGWG6mA-3′b II β IV, VI, X, I, II
  Phi145_37 mtPhi145-2 M.Phi145II Replication 5′-CY6mAG-3′d II α X, I, II, IV, VI, VIII
  1. aMotif identified on two independent SMRT sequencing runs with Phi15 propagated on two separate hosts.
  2. bValidated by restriction analysis using HphI. Phi93_002 and Phi93_039 are 99% and 100% identical to Phi145_002 and Phi145_037 respectively, therefore only the genes from Phi145 were cloned and validated by restriction analysis.
  3. cValidated by restriction analysis using DpnI and DpnII.
  4. dAssigned based on comparative sequence alignment to mtPhi15-1.