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Figure 1 | BMC Genomics

Figure 1

From: iTRAQ-proteomics and bioinformatics analyses of mammary tissue from cows with clinical mastitis due to natural infection with Staphylococci aureus

Figure 1

MS/MS spectrum for the relative quantity of two representative proteins (a) COL1A1 (Accession: tr|F1MGW0|F1MGW0_BOVIN); (b) ITIH4 (Accession: tr|Q5EA67|Q5EA67_BOVIN). The two lines on the top of the peak figure correspond to the peptides sequence (the first line is the positive sequence, the second line is a reverse sequence); Amino acid with bold letters present detected sequence. Amino acid + digital present the decorated aa, while the number shows the quality deviation occurred, such as + 304 denotes the ITRAQ modification is introduced by the ITRAQ reagent. Horizontal axis is the value of m/z and the vertical axis is the normalized intensity. B1, b2, b3,… Y1, y2, y3… correspond to the peak of the detected b and y ions. The peaks corresponding to ITRAQ–114 and ITRAQ-117 mark these 2 pool samples. The intensity of two peaks was used for the quantitative of protein. The peak figure is done by Scaffold software at http://www.proteomesoftware.com/.

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