Figure 3From: A systematic analysis of protein palmitoylation in Caenorhabditis elegansLocomotive behaviour in solution is mostly unaffected by disruption of palmitoylation enzymes. Thrashing assays were performed on animals deficient for DHHC enzymes, either mutant strains (A) or feeding RNAi-treated hypersensitive rrf-3 mutants (C) and compared with wild-type Bristol N2 or empty L4440 vector control respectively. Similarly, thrashing assays were performed on mutant strains (B) and feeding RNAi-treated rrf-3 mutants (D) for the PPT enzymes. (E) Closely related pairs of enzymes were knocked down by feeding RNAi and subjected to thrashing assays. n = 30–288 animals, from 3–14 independent experiments for mutants; and 10–49 animals, from 1–5 independent experiments for RNAi. *p < 0.05 by one-way ANOVA.Back to article page