Figure 2
Identification of novel gene models and non-coding RNAs. A. Of the 819 novel transcriptionally active regions (nTARs) identified, 5 nTARs display similarity with non-coding RNAs and 146 with known proteins (E-value 10-8). B. About 80% of these share significant identity with proteins from other Colletotrichum species. C. One example of a novel transcript that has not been identified by automated annotation of the genomic sequence. Blast analysis revealed a protein of 127 amino acids with similarity (E-value 10-34) to the putative effector ChEC22 from Colletotrichum higginsianum. The green bar highlights the cds of the ChEC22 ortholog, grey bars represent individual RNA-Seq reads. D. Transcript profile of the Chec22 ortholog in C. graminicola. Transcriptional activity at 24-120 hours postinoculation (hpi) correlates with necrotrophic development of C. graminicola in maize leaves.