Figure 1
From: Transcriptome reconstruction and annotation of cynomolgus and African green monkey
The schematic of the strategy for reconstructing and annotating transcriptomes. We leveraged the Tuxedo suite to generate reference-based transcriptome assemblies and used our Multi-Species Annotation (MSA) pipeline for annotation: A) We applied TopHat and Cufflinks to quality-filtered, tissue-specific RNA-seq samples, and generated a combined transcriptome assembly using Cuffmerge. B) We used the MSA pipeline for annotation. This pipeline has three steps: 1) alignment via parallelized iterative BLAST, 2) assignment of gene symbols via homology, and 3) correction and filtering.