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Table 1 Study collection and characteristics of the strains

From: Non-typeable pneumococci circulating in Portugal are of cps type NCC2 and have genomic features typical of encapsulated isolates

CCa Strain Year PFGE MLST Antibiotype (non susceptible to)b CSP/ComDc Capsular region RFLP Capsular region sequenced Analysis by CGH
344 PT944 2001 NT1 344 PG, Ery, Da, Tet, SXT 2/2 A Yes Yes
  LGST142 2000 NT1 344 Ery, Da, Tet, SXT 2/2 A No No
  PT191 2001 NT1 344 PG, Ery, Da, Tet, SXT 2/2 A No No
  PT3412b 2002 NT1 344 Ery, Da, Tet, SXT 2/nd A No No
  PT998 2001 NT1 344 PG, Ery, Tet, SXT 2/nd A No No
  LGST214 2000 ND 344 Ery, Tet, SXT 2/nd A No No
  DCC2367 1999 NT1 344 PG, Tet, SXT 2/2 F Yes No
  PT389 2001 NT1 344 PG, Tet, SXT 2/nd F No No
  PT4427a 2002 NT1 344 PG, Ery, Tet, SXT 2/nd H Yes No
  WL212 2001 NT1 1619 PG, Ery, Da, SXT 2/2 A No Yes
  PT5899 2007 NT1 5220 PG, Ery, Tet, SXT 2/nd nd No Yes
  DCC635 1997 NT2 344 PG, Ery, Da, Tet, SXT 2/2 A No Yes
  WL992 2002 NT3 344 PG, Ery, Da, Tet, SXT 2/2 A No Yes
  PT2987 2002 NT4 344 PG, Ery, Da, Tet, SXT 2/nd A No No
  PT2293b 2001 NT4 344 PG, Ery, Da, Tet, SXT 2/2 E Yes Yes
  PT6317 2007 NT5 344 PG, Ery, Da, Tet, SXT 2/nd nd No Yes
  PT5838b 2007 NT6 344 Ery, Da, Tet, SXT 2/nd nd No Yes
  WL1514 2003 NT7 344 PG, Ery, Da, Tet, SXT 2/2 A No Yes
  PT6318 2007 NT7 4586 PG, Ery, Da, Tet, SXT 2/nd nd No Yes
  PT5269 2006 NT8 344 PG, Ery, Da, Tet, SXT 2/nd nd No Yes
  DCC2879 1999 NT9 897 PG, Ery, Da, Tet, SXT 2/2 A No Yes
  PT1571b 2001 NT10 344 PG, Ery, Da, Tet, SXT 2/2 A No Yes
  PT5727 2006 NT11 344 PG, Ery, Da, Tet, SXT 2/nd nd No Yes
  PT5082a 2003 NT22 344 PG, Ery, Da, Tet, SXT 2/nd I No No
  WL598 2001 NT25 344 PG, Tet, SXT 2/nd F No No
  DCC1795 1998 NT26 1541 PG, Ery, Da, Tet, SXT 2/2 A No Yes
  DCC2435p 1999 ND 344 Ery, Da, Tet, SXT 2/nd A No No
1156 PT268 2001 NT21 1156 PG, Ery, Da, Tet, SXT 1/nd A No No
  PT6210 2007 NT21 4583 PG, Ery, Da, Tet, SXT 1/nd nd No Yes
  PT2687b 2001 NT22 1156 PG, Ery, Da, Tet, SXT 1/nd A No No
  PT5561 2006 NT22 1156 PG, Ery, Da, Tet, SXT 1/nd nd No Yes
  PT4014 2002 NT22 1153 PG, Ery, Da, Tet, SXT 1/1 C No Yes
  PT4222 2002 NT24 1156 PG, Ery, Da, Tet, SXT 1/1 A No No
  PT5002 2003 NT24 1156 Ery, Da, Tet 1/1 A No Yes
  PT1493 2001 NT24 1617 PG, SXT 1/1 A Yes Yes
  WL352.1 2001 NT24 1703 PG, SXT 1/1 A No Yes
  PT3201 2002 NT24 1153 PG, Ery, Da, Tet, SXT 1/1 C Yes Yes
  PT6209b 2007 NT24 4583 PG, Ery, Da, Tet 1/nd nd No Yes
  PT2322 2001 ND 1153 PG, Ery, Da, Tet, SXT 1/nd C No No
320 PT1804b 2001 NT19 888 PG, SXT 1/1 A Yes Yes
1540* PT1718 2001 NT12 1540 SXT 1/4 A Yes Yes
1278* PT4812 2003 NT22 1278 PG, SXT 1/1 A Yes Yes
941 DCC2787 1999 NT13 941 SXT 2/2 B Yes Yes
  WL165b 2001 NT13 1704   2/2 B No Yes
  DCC2648 1999 NT14 941 SXT 2/2 B No Yes
448 WL850a 2002 NT15 448   2/2 B Yes Yes
  WL1084 2002 NT15 448   2/2 B No No
  PT2417 2001 NT15 448 PG, SXT 2/nd B No No
  WL108 2001 NT16 448   2/nd nd No Yes
1618 PT673 2001 NT17 1618 PG, Ery 1/1 D Yes Yes
  WL402.1b 2001 NT17 1618 PG, Ery, Da, Tet, SXT 1/1 D No Yes
1705* WL977 2002 NT23 1705 PG, SXT 1/1 G Yes Yes
  1. a – clonal complex (CC); singleton (*); b – penicillin G (PG), erythromycin (Ery), clindamycin (Da), tetracycline (Tet), and trimethoprim sulfamethoxazole (SXT); c – ComD2 had an E151K substitution and ComD4 had an M77I and an E151K substitutions, both outside the sensor domain of ComD; nd – not determined.
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