KvarQ scanning time for all 206 polymorphic positions with respect to average sequence coverage of the MTBC genome per fastq file. Each dot represents one isolate (i.e. fastq file). Scanning time was found to be 112 seconds/100× coverage. Eleven isolates of 880 were excluded due to variable read lengths (difficult calculation of total base output). Three additional isolates were excluded because of file sizes larger than 10 GB.