DNA methylation profiles of Meis1 and tissue-specific expression pattern. A) Bisulfite converted DNA was cloned and sequenced. Each line represents an individual PCR product. The annotated promoter CpG island was assayed for DNA methylation. Unfilled circles represent unmethylated cytosines at CpG dinucleotides and filled circles represent methylated cytosines. B) 1% agarose gel of PCR amplicons (cDNA) generated from liver, brain, and heart mRNA from four F1 animals used in this study (A0690. A0695, A0716, A0727). Igf2R is expressed in all three tissues, but Meis1 is not expressed. Ladder = 100 bp DNA Ladder; Positive control = fibroblast cDNA.