Figure 4

Localization and expression of PRDX5 and PHGPx before- and after-capacitation in porcine spermatozoa. (A and E) Images of PRDX5 and PHGPx before capacitation (green). (B and F) Merged image of the nucleus (DAPI, blue) and acrosome (lectin PNA, red) with PRDX5 and PHGPx before-capacitation, respectively (green). (C and G) Images of PRDX5 and PHGPx after-capacitation (green). (D and H) Merged image of nucleus (DAPI, blue) and acrosome (lectin PNA, red) with PRDX5 and PHGPx after-capacitation, respectively (green). Images were obtained using a Nikon TS-1000 microscope and NIS Elements image software (Nikon, Japan). Bar =10 μm. (I) Ratios of PRDX5 and PHGPx [optical density (OD x mm)/α-tubulin (OD x mm)] before- and after-capacitation. Data represent mean ± SEM, n =3. Proteins expression ratios with superscripts were significantly different (*P < 0.05). (J) PRDX5 and PHGPx were probed with anti-PRDX5 and anti-PHGPx antibody.