Table 10 Validation of microarray results with real time PCR
| Â | Â | MDS | MDEL | SUCLA-2 | ||
|---|---|---|---|---|---|---|
Gene | Description | FC array | FC Fluidigm | FC array | FC Fluidigm | FC Fluidigm |
FBP2 | fructose-1,6-bisphosphatase 2 | -9.1 | -23.4 | ns | -1.18 | -1.59 |
ATP2A1 | ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 | -9.1 | -13.3 | ns | 1.07 | 1.31 |
PFKFB3 | Phosphofructokinase-biphosphatase-3 | -4.6 | -1.7 | ns | -1.14 | -2.32 |
LDHA | Lactate-dehrydrogenase-A | -4.1 | -7.9 | ns | -1.67 | -2.85 |
ADCK3 | aarF-domain-containing kinase 3 | -3.4 | -2.7 | ns | -1.21 | 1.19 |
CYTB | Cytochrome b | -3 | -7.63 | ns | -1.32 | 3.44 |
GDF15 | Growth Differentiation Factor 15 | 188 | 845 | 6 | 154.6 | 2.46 |
TRIB3 | Tribbles homolog 3 | 47 | 141.3 | 5 | 29.5 | 2.92 |
TNNT2 | Troponin T2, cardiac | 21 | 35.9 | ns | 7.3 | 6.62 |
COL19A1 | Collagen type XIX | 11 | 100.6 | ns | 19.7 | 1.66 |
FGF21 | Fibroblast Growth Factor 21 | 10.8 | 1281.1 | ns | 286.8 | - |
HMGCS2 | 3-hydroxy-3-methylglutaryl-CoA synthase 2 | 6.2 | 9.2 | 2.7 | 4.99 | 66.85 |
PTPRF | Receptor-type tyrosine-protein phosphatase F | 6.1 | 9.6 | ns | 2.09 | 3.2 |
G0S2 | G0/G1 switch regulatory protein | 6.1 | 1.91 | 4.8 | 2.21 | 1.5 |
SHMT2 | Serine hydroxyl-methyltransferase 2 | 3.4 | 4.8 | ns | 1.84 | -1.06 |
ASS1 | argininosuccinate synthase-1 | 4.7 | 3.1 | ns | 1.53 | 3.52 |
TP53I3 | TP53 induced gene 3 | 3.5 | 2.42 | ns | -1.30 | 1.54 |
DPYSL4 | Dihydropyrimidinase-related protein 4 | 2.6 | 5.48 | 3.1 | 1.30 | - |
TP53 | Â | ns | 1.8 | ns | 1.01 | 1.61 |
PPARG | Peroxisome proliferator-activated receptor gamma | ns | -1.02 | ns | -1.10 | 1.54 |
SUCLA2 | succinate-CoA ligase, ADP-forming, beta subunit | ns | -2.28 | ns | -1.29 | -1.35 |
TK2 | Tymidine kinase 2 | ns | -1.19 | ns | -1.24 | 1.37 |