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Table 11 qRT-PCR primers

From: Transcriptomic profiling of TK2 deficient human skeletal muscle suggests a role for the p53 signalling pathway and identifies growth and differentiation factor-15 as a potential novel biomarker for mitochondrial myopathies

Gene name

Gene symbol

Taqman gene expression assays

TATA box binding protein

TBP

Hs99999910_m1

Hypoxanthine phosphoribosyltransferase 1

HPRT1

Hs02800695_m1

Actin, beta

ACTB

Hs01060665_g1

Desmin

DES

Hs00157258_m1

3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial)

HMGCS2

Hs00985427_m1

Serine hydroxymethyltransferase 2 (mitochondrial)

SHMT2

Hs00193658_m1

Argininosuccinate synthase 1

ASS1

Hs01597989_g1

Tumor protein p53 inducible protein 3

TP53I3

Hs00153280_m1

Complement component 3

C3

Hs00163811_m1

Tumor protein p53

TP53

Hs01034249_m1

Hemoglobin, beta

HBB

Hs00758889_s1

G0/G1switch 2

G0S2

Hs00274783_s1

Collagen, type XIX, alpha1

COL19A1

Hs00156940_m1

Protein tyrosine phosphatase, receptor type, F

PTPRF

Hs00892965_m1

Peroxisome proliferator-activated receptor gamma

PPARG

Hs01115513_m1

Fructose-1,6-bisphosphatase 2

FBP2

Hs00427791_m1

Cytochrome b

MT-CYB

Hs02596867_s1

Lactate dehydrogenase A

LDHA

Hs00855332_g1

aarF domain containing kinase 3

ADCK3

Hs00220382_m1

Troponin T type 2 (cardiac)

TNNT2

Hs00165960_m1

ATPase, Ca++ transporting, cardiac muscle, fast twitch 1

ATP2A1

Hs01092295_m1

Dihydropyrimidinase-like 4

DPYSL4

Hs01067761_m1

Fibroblast growth factor 21

FGF21

Hs00173927_m1

Tribbles homolog 3 (Drosophila)

TRIB3

Hs01082394_m1

Growth differentiation factor 15

GDF15

Hs00171132_m1

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3

PFKFB3

Hs00998700_m1

Succinate-CoA ligase, ADP-forming, beta subunit

SUCLA2

Hs00605838_g1

Thymidine kinase 2, mitochondrial

TK2

Hs00177950_m1