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Table 2 Sequences and mapping statistics

From: Comparison of library preparation methods reveals their impact on interpretation of metatranscriptomic data

  Raw reads (millions) % rRNAa Cleaned readsb(millions) % mapped readsc % duplication ratesd
Library name replicate replicate replicate replicate replicate replicate replicate replicate replicate replicate
1 2 1 2 1 2 1 2 1 2
TS_L.L 0.724 0.747 0.14 0.23 0.720 0.743 77.2 72.3 2.19 5.84
ENC_L.L 0.764 0.739 36.6 37.96 0.481 0.454 67.1 51.2 5.52 13
OV_L.L 0.809 0.792 0.2 0.39 0.802 0.784 65.3 61 2.3 2.45
SMART_L.L 0.904 0.697 2.9 2.88 0.837 0.650 62.9 63.7 8.76 7.62
TS_L.L control 22.161 19.845 95.6 95.4 0.960 0.885 71.2 71.4 8.47 6.92
OV_L.L control 21.989 17.513 71 78.,5 6.302 3.676 73 74.4 1.01 0.57
SMART_L.L control 24.806 18.798 95 95.1 1.146 0.831 51.5 51.9 54 61.6
TS_MIX 0.754 0.769 0.21 2.27 0.748 0.747 73.1 72.1 1.23 1.59
ENC_MIX 0.749 0.732 37 36.61 0.470 0.461 66.5 66 7.4 5.58
OV_MIX 0.864 1.177 0.9 0.6 0.849 1.161 55.1 54.7 0.93 0.85
SMART_MIX 0.824 0.726 0.77 0.96 0.809 0.702 65.7 66.9 6.5 7.03
TS_MIX control 20.257 30.693 93 94.1 1.393 1.775 70.4 71.1 3.24 3.,28
OV_MIX control 19.510 17.984 84 82 2.983 3.215 75.2 70.4 1.78 0.39
SMART_MIX control 14.699 18.603 93.5 93.5 0.915 1.160 63.9 61.9 23 39
  1. _L.L: library prepared from L. lactis depleted RNA.
  2. _L.L control: library prepared from L. lactis total RNA.
  3. _MIX: library prepared from the MIX depleted RNA.
  4. _MIX control: library prepared from the MIX total RNA.
  5. aproportion of rRNA reads detected in the raw reads.
  6. bnumber of sequences remaining after the data quality control pipeline treatment applied on raw reads.
  7. cproportion of cleaned reads uniquely mapped on CDS sequences.
  8. dduplication rate estimated on 100 000 cleaned reads.