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Table 1 BLASTX of combined FW&SW assembled contigs mapped to Oreochromis niloticus , Danio rerio and Homo sapiens protein databases

From: Differential transcriptomic analyses revealed genes and signaling pathways involved in iono-osmoregulation and cellular remodeling in the gills of euryhaline Mozambique tilapia, Oreochromis mossambicus

Database

BLASTX E-value range

Number of mapped contigs (Percentage of total selected contigs)*

Number of mapped unique identifier in protein database (Percentage of sequences in respective database)**

Oreochromis niloticus (NCBI Nile tilapia protein database release 101 containing 45443 sequences)**

0

9845 (12.8%)

8830 (19.4%)

≤1E-100

15733 (20.4%)

12946 (28.5%)

≤1E-50

23556 (30.6%)

16433 (36.2%)

≤1E-20

32541 (42.2%)

18474 (40.7%)

≤1E-05

38925 (50.5%)

19510 (42.9%)

Danio rerio (NCBI zebrafish protein database release 103 containing 42940 sequences)**

0

5667 (7.35%)

4989 (11.6%)

≤1E-100

10410 (13.51%)

8594 (20.0%)

≤1E-50

16452 (21.35%)

12133 (28.3%)

≤1E-20

24150 (31.34%)

14779 (34.4%)

≤1E-05

31775 (41.23%)

16397 (38.2%)

Homo sapiens (Direct BLASTX to the NCBI human non-redundant database)**

0

4418 (5.7%)

4063

≤1E-100

8583 (11.1%)

7796

≤1E-50

14018 (18.2%)

12317

≤1E-20

20785 (27.0%)

17022

≤1E-05

28446 (36.9%)

21075

  1. *Total selected contigs (average coverage ≥20) =77062. Percentage of total selected contigs = (number of contigs/77062) x 100%.
  2. **Percentage of Nile tilapia protein database = (Number of unique identifiers/45443) x 100%. Percentage of zebrafish protein database = (Number of unique identifiers/42940) x 100%. Direct BLASTX to the NCBI human non-redundant database were used and information on exact number of sequence is not available, therefore percentage of human nr database is not determined.