qRT-PCR validation of selected DEGs. (A) The x-axis represents five developmental periods, and the y-axis indicates the relative expression value by qRT-PCR analysis. Data shown are means of three biological replicates. Error bars indicate ± SD. (B) Comparison of gene expression ratios observed by microarray and qRT-PCR analyses. Data are from 12 probe sets at five time points between TM-1 and the im mutant. The microarray log2(expression ratio) values (x-axis) are plotted against the log2(expression ratio) obtained by qRT-PCR (y-axis). Asterisks indicate significant differences of expressional level (Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001) between TM-1 and the im mutant at same developmental stage.