Table 2 Quantitative RT-PCR compared to RNA-seq data for 16 genes a
RNA-seq | RT-PCR | |||||
|---|---|---|---|---|---|---|
Gene | Base mean in N2 | Base mean in wrn-1 | Fold difference | ΔCt | Fold difference | P-value |
sodh-1 | 465 | 5021 | +10.79 | 3.02 | +8.09 | <0.001 |
arrd-24 | 4 | 32 | +7.80 | none | N/A | N/A |
gpx-3 | 24 | 146 | +6.21 | 1.07 | +3.24 | 0.0012 |
nas-3 | 49 | 220 | +4.51 | 1.78 | +3.43 | <0.001 |
daf-9 | 168 | 605 | +3.60 | 1.48 | +2.78 | <0.001 |
asah-1 | 393 | 1200 | +3.06 | 0.72 | +1.65 | 0.0008 |
acs-2 | 594 | 1277 | +3.30 | 1.80 | +3.48 | <0.001 |
acs-14 | 763 | 2289 | +3.00 | 0.52 | +1.43 | 0.0307 |
ech-9 | 155 | 449 | +2.89 | 1.94 | +3.84 | 0.0006 |
Y46G5.20 | 1182 | 381 | -3.10 | -2.05 | -4.14 | 0.0001 |
cyp-33e2 | 1708 | 548 | -3.12 | -2.70 | -6.52 | <0.001 |
cyp-34a9 | 987 | 289 | -3.41 | -2.05 | -4.15 | <0.001 |
pes-8 | 256 | 66 | -3.84 | -2.06 | -4.16 | 0.0204 |
ugt-33 | 269 | 69 | -3.90 | -1.67 | -3.18 | 0.0008 |
lys-8 | 3405 | 155 | -21.93 | -4.45 | -21.87 | <0.001 |
nlp-32 | 135 | 5 | -28.69 | none | N/A | N\A |
tba-1 b | 12597 | 10618 | -1.19 | -0.12 | -1.09 | 0.7083 |