Skip to main content

Table 2 List of membrane proteins and N-glycosylation sites associated with microglial physiology

From: Characterization of the membrane proteome and N-glycoproteome in BV-2 mouse microglia by liquid chromatography-tandem mass spectrometry

Group Protein type Representing name Gene symbol Protein name N-glycosites (*Noble site) Max. sequence coverage
Microglia marker   CD11b Itgam Integrin alpha- 58 N, 391 N, 696 N, 734 N, 907 N, 941 N, 1022 N, 1045 N 13.2% (B1)
CD18 Itgb2 Integrin beta-2 51 N, 502 N, 626 N, 644 N 29.8% (B2)
CD11c Itgax Integrin alpha-X 393 N* Glycol-only
  Ptprc Receptor-type tyrosine-protein phosphatase C 210 N*, 215 N*, 247 N*, 268 N*, 279 N*, 290 N*, 304 N*, 311 N*, 322 N*, 347 N*, 384 N*, 427 N*, 446 N*, 489 N*, 1237 N* 27.9% (B2)
CD68 Cd68 Macrosialin 129 N*, 134 N*, 169 N*, 178 N, 260 N 16.1% (B2)
F4/80 antigen Emr1 EGF-like module-containing mucin-like hormone receptor-like 1 405 N*, 413 N*, 417 N*, 498 N 21.3% (B2)
Iba1 Aif1 Allograft inflammatory factor 1 - 8.2% (B2)
Ion channel Calcium channel TRPs Trpm7 Transient receptor potential cation channel subfamily M member 7 - 1.7% (B1)
Trpc2 Short transient receptor potential channel 2 - 0.8% (B1)
Trpc4 Short transient receptor potential channel 4 - 0.8%(B1)
Trpv2 Transient receptor potential cation channel subfamily V member 2 567 N* 33.1%(B1)
Potassium channel Inward rectifier potassium channels Kcnj2 Inward rectifier K(+) channel Kir2.1 - 3.3%(B1)
BK channels Kcnma1 Calcium-activated potassium channel subunit alpha-1 - 1.6%(B2)
Kcnu1 Calcium-activated potassium channel subunit alpha-3 339 N* Glyco-only
Kcnmb3 Calcium-activated potassium channel subunit beta-3 - 4.6%(B2)
Kcnab2 Voltage-gated potassium channel subunit beta-2 - 8.6%(B2)
anion channels CLIC-1 chloride channels Clic1 Chloride intracellular channel protein 1 - 60.6%(B2)
other channels Proton channels Hvcn1 Hydrogen voltage-gated channel 1 - 13.4%(B1)
Neuro-Trans-mitter Receptor Purino ceptors P2X4 P2rx4 P2X purinoceptor 4 75 N, 131 N, 153 N*, 184 N, 208 N 21.6%(B2)
P2X7 P2rx7 P2X purinoceptor 7 74 N, 187 N, 202 N, 213 N 12.8%(B1)
P2Y6, P2Y10 P2ry6 P2Y purinoceptor 6 - 3.7%(B1)
  A630033H20Rik Novel protein similar to purinergic receptor P2Y G-protein coupled 10 (P2ry10) - 3.7%(B1)
Glutamate receptor AMPA receptors Gria2 AMPA-selective glutamate receptor 2 - 1.7%(B2)
Metabotropic glutamate receptors Grm1 Isoform 1 of Metabotropic glutamate receptor 1 - 2%(B1)
GABA receptors Gabra5 Gamma-aminobutyric acid receptor subunit alpha-5 - 1.7%(B1)
Neuro-hormones and neuro-modu-lators receptor Glucocorticoid receptors Glucocorticoid receptor Nr3c1 Glucocorticoid receptor - 4.2%(B1)
Opioid receptors   Sigmar1 Sigma 1-type opioid receptor - 43.5%(B2)
Ogfr Opioid growth factor receptor - 17.1%(B2)
Cytokine receptors TNF-alpha receptors Tnfr2 TNF-alpha receptor 2 - 9.5%(B1)
Tnfrsf1b TNF receptor superfamily member 1B - 9.5%(B1)
Tnfrsf26 TNF receptor superfamily member 26 136 N* 14.2%(B1)
Interleukin receptors Il2rg Interleukin 2 receptor, gamma chain 96 N, 159 N*, 164 N*, 306 N* 5.1%(B1)
Il10rb Interleukin-10 receptor subunit 2 51 N* 11.4%(B1)
Il13ra1 Interleukin-13 receptor subunit alpha-1 262 N Glyco-only
Il6st Interleukin-6 receptor subunit beta 43 N Glyco-only
Il10ra Interleukin-10 receptor subunit alpha 113 N* Glyco-only
Il4ra Isoform 1 of Interleukin-4 receptor subunit alpha 163 N Glyco-only
Csf2rb Cytokine receptor common subunit beta 141 N* 2.7%(B1)
Other receptor system   Notch receptors Notch2 neurogenic locus notch homolog protein 2 - 3.3%(B1)
Complement receptors C5ar1 Complement component 5a receptor 1 - 9.4%(B1)
Macrophase colony-stimulating factor receptors Csf1r Macrophage colony-stimulating factor 1 receptor - 16.6%(B1)
Csf2ra Granulocyte-macrophage colony-stimulating factor receptor subunit alpha 132 N*, 165 N, 269 N* Glyco-only
Formyl peptide receptors Fpr1 fMet-Leu-Phe receptor - 9.3%(B2)
CD200 receptors Cd200r1 CD200 cell surface glycoprotein receptor 93 N, 192 N* 5.5%(B1)
Cd200r4 CD200 cell surface glycoprotein receptor-like 4 - 12.6%(B1)