Expression pattern confirmed by qRT-PCR and comparison with sequencing data. Relative expression pattern of six different conserved miRNAs among various libraries are confirmed by qRT-PCR results by 2-∆∆Ct method. For visualization, log10 method is applied to compute the TPM data of sequencing results. The paragraph A to F represent the expression profiles of miR166a, miR4376-5p, miR156k, miR396b-3p, miR164c* and miR159b-3p in sequence. Y-axis represents the expression level and X-axis represents different libraries. Blue lines represent the q-PCR data and red lines represent the sequencing data. The line charts indicate the similar expression patterns with miRNA sequencing.