Table 2 Target genes of conserved miRNAs (excerpted*)

miR1061-3p

Pbr041100.1

Plant-pathogen interaction(7.190677e-06)

Pbr025269.1

Plant-pathogen interaction(7.190677e-06)

Pbr019706.1

Plant-pathogen interaction(7.190677e-06)

Pbr014433.1

Plant-pathogen interaction(7.190677e-06)

Pbr010237.1

Plant hormone signal transduction(0.01510044) Plant-pathogen interaction(7.190677e-06)

Pbr002521.1

Plant hormone signal transduction(0.01510044) Plant-pathogen interaction(7.190677e-06)

Pbr029017.1

Phenylalanine, tyrosine and tryptophan biosynthesis(1.488743e-06) Metabolic pathways(0.7902142) Other glycan degradation(0.855243) Fructose and mannose metabolism(0.6615606) Glycosphingolipid biosynthesis - ganglio series(0.5541742) Sphingolipid metabolism(0.1582752) Lysosome(0.9793836) Amino sugar and nucleotide sugar metabolism(0.6427769) Glycosaminoglycan degradation(0.4927528) Ribosome(0.987106) Galactose metabolism(0.2583273) Biosynthesis of secondary metabolites(0.9498542)

Pbr031602.1

Mineral absorption(0.00709937)

Pbr029046.1

Mineral absorption(0.00709937)

Pbr021467.1

Mineral absorption(0.00709937)

Pbr002476.1

Mineral absorption(0.00709937)

Pbr012616.2

Metabolic pathways(0.7902142) Folate biosynthesis(0.1157641) ABC transporters(0.06561596) Arginine and proline metabolism(0.1871821) GABAergic synapse(0.9817247) Nitrogen metabolism(0.5698157) Polyketide sugar unit biosynthesis(0.3271947) Alanine, aspartate and glutamate metabolism(0.9892745) Glyoxylate and dicarboxylate metabolism(0.9626975) Glutamatergic synapse(0.9998555) Streptomycin biosynthesis(0.5326464) Microbial metabolism in diverse environments(0.9297261) Biosynthesis of secondary metabolites(0.9498542) Two-component system(0.2053418)

Pbr021166.1

Influenza A(0.2265907) Tuberculosis(0.08865112) Leishmaniasis(0.04564140) Chagas disease (American trypanosomiasis)(0.02067325) Plant-pathogen interaction(7.190677e-06) Toxoplasmosis(0.2633427) Toll-like receptor signaling pathway(0.05315244) Neurotrophin signaling pathway(0.05126187) Apoptosis(0.06810953) NF-kappa B signaling pathway(0.073712) Measles(0.06773311) Pertussis(0.03473645)

Pbr040822.1

Flavonoid biosynthesis(0.599654) Metabolic pathways(0.7902142) Biosynthesis of secondary metabolites(0.9498542)

Pbr040817.1

Flavonoid biosynthesis(0.599654) Metabolic pathways(0.7902142) Biosynthesis of secondary metabolites(0.9498542)

Pbr021205.1

Ethylbenzene degradation(0.369114) Leishmaniasis(0.04564140) Chagas disease (American trypanosomiasis)(0.02067325) Plant-pathogen interaction(7.190677e-06) Toxoplasmosis(0.2633427) Neurotrophin signaling pathway(0.05126187) Microbial metabolism in diverse environments(0.9297261) NF-kappa B signaling pathway(0.073712) Measles(0.06773311) Tyrosine metabolism(0.3092827) Influenza A(0.2265907) Aminobenzoate degradation(0.3980524) Tuberculosis(0.08865112) Metabolic pathways(0.7902142) Fructose and mannose metabolism(0.6615606) Limonene and pinene degradation(0.656067) Benzoate degradation(0.3951967) Toll-like receptor signaling pathway(0.05315244) Apoptosis(0.06810953) Pertussis(0.03473645) Pyrimidine metabolism(0.0004838167)

Pbr035730.1

Cytosolic DNA-sensing pathway(6.107676e-13) Metabolic pathways(0.7902142) Plant-pathogen interaction(7.190677e-06) RNA polymerase(1.034169e-09) Pyrimidine metabolism(0.0004838167) Epstein-Barr virus infection(8.479755e-05) Huntington’s disease(8.38133e-08) Purine metabolism(0.0001980336)

Pbr010706.1

Circadian rhythm - mammal(0.3646747) Wnt signaling pathway(0.3688827) Protein processing in endoplasmic reticulum(0.9999496) TGF-beta signaling pathway(0.9755566) Ubiquitin mediated proteolysis(0.321184) Oocyte meiosis(0.9154166) Cell cycle(0.9997656) Cell cycle - yeast(0.5227999) Herpes simplex infection(0.9478489)

Pbr026497.1

Cell cycle - yeast(0.5227999)

miR1121

Pbr018590.1

Metabolic pathways(0.7902142) Glycosylphosphatidylinositol(GPI)-anchor biosynthesis(0.1935023)

miR1318

Pbr027745.1

Valine, leucine and isoleucine degradation(0.853519) Propanoate metabolism(0.7921382) Metabolic pathways(0.7902142) Lysine biosynthesis(0.3398862) Glycine, serine and threonine metabolism(0.8336738) Tryptophan metabolism(0.4881696) Arginine and proline metabolism(0.1871821) Pyruvate metabolism(0.9296067) Glycolysis / Gluconeogenesis(0.9894463) beta-Alanine metabolism(0.8613309) Fatty acid metabolism(0.830646) Histidine metabolism(0.8285719) Glycerolipid metabolism(0.707723) Lysine degradation(0.3979612) Biosynthesis of secondary metabolites(0.9498542) Ascorbate and aldarate metabolism(9.178918e-07)

Pbr009875.1

Spliceosome(0.338066)

Pbr020008.1

Regulation of autophagy(0.2863020)

Pbr013669.1

Plant-pathogen interaction(7.190677e-06)

Pbr009166.1

Plant hormone signal transduction(0.01510044)

Pbr036152.1

Phenylpropanoid biosynthesis(0.1081946) Metabolic pathways(0.7902142) Biosynthesis of secondary metabolites(0.9498542) Phenylalanine metabolism(0.1547410)

Pbr038845.1

Naphthalene degradation(0.930259) Aminobenzoate degradation(0.3980524) Chloroalkane and chloroalkene degradation(0.9450628) Polycyclic aromatic hydrocarbon degradation(0.4501106) Microbial metabolism in diverse environments(0.9297261) Bisphenol degradation(0.8789056) Limonene and pinene degradation(0.656067) Biosynthesis of secondary metabolites(0.9498542)

Pbr015038.1

Metabolic pathways(0.7902142) Citrate cycle (TCA cycle)(0.997454) Microbial metabolism in diverse environments(0.9297261) Biosynthesis of secondary metabolites(0.9498542)

Pbr031471.1

GABAergic synapse(0.9817247)

Pbr013295.1

Circadian rhythm - plant(0.924008)

Pbr026842.1

Alanine, aspartate and glutamate metabolism(0.9892745) Bacterial secretion system(0.8448431) Metabolic pathways(0.7902142) Protein export(0.6659996) Pyrimidine metabolism(0.0004838167)

Pbr004124.1

Alanine, aspartate and glutamate metabolism(0.9892745) Bacterial secretion system(0.8448431) Metabolic pathways(0.7902142) Protein export(0.6659996) Pyrimidine metabolism(0.0004838167)

miR160a

Pbr042396.1

Plant hormone signal transduction(0.01510044)

Pbr039066.1

Plant hormone signal transduction(0.01510044)

Pbr037271.1

Plant hormone signal transduction(0.01510044)

Pbr016467.1

Plant hormone signal transduction(0.01510044)

Pbr008550.1

Plant hormone signal transduction(0.01510044)

Pbr008404.1

Plant hormone signal transduction(0.01510044)

Pbr008352.1

Plant hormone signal transduction(0.01510044)

Pbr008195.1

Plant hormone signal transduction(0.01510044)