Table 1 Porcine specific globin oligonucleotides used in RNase H-mediated globin reduction assay
From: Increasing gene discovery and coverage using RNA-seq of globin RNA reduced porcine blood samples
Gene | Sequence | Final Conc. (10X) |
|---|---|---|
HBA | 5′- GAT CTC CGA GGC TCC AGC TTA ACG GT -3′ | 7.5 uM |
5′- TCA ACG ATC AGG AGG TCA GGG TGC AA -3′ | 7.5 uM | |
HBB | 5′- AGG GGA ACT TAG TGG TAC TTG TGG GC -3′ | 30 uM |
5′- GGT TCA GAG GAA AAA GGG CTC CTC CT -3′ | 30 uM |