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Table 1 The REs recognition sites and tag numbers of RAD and 2b-RAD in simulated species

From: An improved 2b-RAD approach (I2b-RAD) offering genotyping tested by a rice (Oryza sativa L.) F2 population

Species Size (G) EcoRI SbfI HindIII BsaXI AlfI Version
Arabidopsis thaliana 0.12 37057 627 67057 39826 12601 TAIR9
Cucumis sativus 0.24 69944 1197 94344 65249 23762 v2.3
Theobroma cacao 0.33 90253 1572 173770 81810 39800 CIRAD_v0.9
Oryza sativa 0.37 88471 5541 110084 180848 83465 IRGSP-7.0
Vitis vinifera 0.49 143785 2594 228694 155893 62070 Genoscope
Cajanus cajan 0.61 161249 1652 232261 162320 62119 v1.0
Solanum tuberosum 0.73 233925 2957 273063 212095 70042 v3.4
Sorghum bicolor 0.74 200543 11693 308083 379985 151244 phytozome_v7.0
Glycine max 0.97 309451 3844 422507 289940 140204 phytozome_v7.0
Zea mays 2.06 490315 50773 768456 1151014 444673 AGI_5a
Total 6.65 1824993 82450 2678319 2718980 1089980  
Average 0.67 182499 8245 267831 271898 108998  
Average tag number   364998 16490 535663 271898 108998  
Drosophila melanogaster 0.17 44863 2948 45913 58189 50403 RGSC3.4
Apis mellifera 0.23 97174 551 49977 58053 21237 AnoCar2.0
Anopheles gambiae 0.27 53807 3164 87297 100574 80148 Galgal4
Takifugu rubripes 0.39 75238 20718 95182 210480 103943 FUGU4
Bombyx mori 0.48 112113 2041 121288 96793 47329 AgamP3
Gallus gallus 1.05 277001 48010 403125 453890 400359 NCBIM37
Danio rerio 1.41 255801 30677 427420 426194 339437 v1.0
Mus musculus 2.72 758802 60302 842075 1331056 607106 2.0
Rattus norvegicus 2.72 817664 58283 818955 1257212 595706 r5.27
Homo sapiens 3.10 778227 77095 837133 1292381 583536 v2.0
Total 12.54 3270690 303789 3728365 5284822 2829204  
Average 1.17 327069 30378 372836 528482 282920  
Average tag number   654138 60758 745673 528482 282920