An improved 2b-RAD approach (I2b-RAD) offering genotyping tested by a rice (Oryza sativa L.) F2 population

BMC Genomics

Table 2 The combinations of 4 repeated female

Reads number	Depth	Combination	Covered sites	Coverage rate
2333752	12.8	fe-1	151387	83.71%
2225621	12.2	fe-2	151352	83.69%
1575530	8.5	fe-3	150720	83.34%
2456057	13.4	fe-4	156650	86.62%
3909282	21.6	fe-3 + fe-1	178654	98.79%
3801151	21.0	fe-3 + fe-2	177239	98.00%
4031587	22.3	fe-3 + fe-4	178946	98.95%
6134903	33.9	fe-3 + fe-1 + fe-2	180098	99.59%
8590960	47.5	fe-3 + fe-1 + fe-2 + fe-4	180104	99.59%

“fe” means female parent; “reads number” means the reads which were able to be mapped to reference; “depth” equals to reads number divided by the total expected BsaXI recognition sites ; “covered sites” means the BsaXI sites in reference which was covered by high quality reads; “coverage rate” equals to the “covered sites” divided by the total expected BsaXI recognition sites.

ISSN: 1471-2164