Figure 5From: De-novo assembly of mango fruit peel transcriptome reveals mechanisms of mango response to hot water treatmentValidation of RNA-seq results by means of qRT-PCR. Ten differentially expressed genes (two from each of clusters 1 to 5) were examined by RNA-seq and qRT-PCR at four different time points after HWB treatment: A, B (cluster 1); C, D (cluster 2); E, F (cluster 3); G, H (cluster 4); and I, J (cluster 5). Values were normalized to the values obtained with untreated mango fruit samples at 0Â h and the proportional fold-change (FC) was calculated. Expression data are means of two replicates.Back to article page