Figure 1

Schematic overview of the drug target selection criteria. Genome annotations information for S. pneumoniae R6, S. pneumoniae TIGR4, H. influenzae 86 028NP, H. influenzae Rd KW20, and M. catarrhalis BBH18 were updated using RAST. The proteins with updated annotations were then clustered into putative orthologous groups using OrthoMCL, and their subcellular localizations predicted in various publicly available tools. ESSENTIALS was used to analyse various transposon mutant libraries and predict the essentiality metric for each ORF. Comparing the ensuing essential genes with the catalogue of human gut microbial genes, as well as with the human genome helped to eliminate genes with conserved orthologs, and subsequently prioritize potential drug targets.