From microbial gene essentiality to novel antimicrobial drug targets

BMC Genomics

Table 3 Drug target in vivo validation summary

Compound	Amount on disc (μg)	MIC μg/ml; Std. Dev. [Inhibition area on disk diffusion assay]
		*S. pneumoniae*	H. influenzae	M. catarrhalis
5,5′-dithiobis(2-nitrobenzoate) (CAS 69-78-3)	1,000	2,500; 0 [4 mm*]	781; 313 [none]	319; 303 [none]
1-methyluric acid (CAS 708-79-2)	1,000	>312.5 [6 mm]	>312.5 [none]	>312.5 [none]
5′deoxyadenosine (CAS 4754-39-6)	1,000	78.1; 0 [6 mm]	205; 132 [5 mm*]	29.3; 11 [12 mm]
(R)-6-fluoromevalonate diphosphate (CAS 2822-77-7)	1,000	26.6; 11.5 [12 mm]	4,167; 1443 [none]	>5,000; 0 [none]
(R)-6-fluoromevalonate diphosphate (CAS 2822-77-7)	100	26.6; 11.5 [4 mm*]	4,167; 1443 [none]	>5,000; 0 [none]

Diameter of the clearance zone after normal incubation represents the inhibition area on disk. Concentrations showing delayed growth are denoted by an asterisk (*).
Std. Dev. = Standard deviation.

ISSN: 1471-2164