Comparison of three next-generation sequencing platforms for metagenomic sequencing and identification of pathogens in blood

Table 1 Roche-454 read statistics for DENV-1 at multiple titrations

Dengue-1 pfu/mL	Estimated max. genome copies/mL	Reads mapped per 100,000	Fraction of reference covered	Average C_t	Contig(s) by de novo assembly?	Fraction of reference covered
1X10²	1X10⁴ – 1.7X10⁴	0	0	NA	N	0
1X10^2.3	2X10⁴ – 3.4X10⁴	0	0	27.65	N	0
1X10^2.5	3.2X10⁴ – 5.4X10⁴	0.34	0.05	ND	N	0
1X10^2.5	3.2X10⁴ – 5.4X10⁴	1.82	0.31	27.03	Y	0.04
1X10³	1X10⁵ – 1.7X10⁵	3.4	0.4	25.66	Y	0.20
1X10³	1X10⁵ – 1.7X10⁵	4.7	0.72	ND	ND	ND
1X10⁴	1X10⁶ – 1.7X10⁶	34.2	0.92	21.79	Y	0.76
1X10⁵	1X10⁷ – 1.7X10⁷	190.9	0.99	18.05	Y	0.99
1X10⁶	1X10⁸ – 1.7X10⁸	227.1	0.99	14.56	Y	0.99

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