Comparison of three next-generation sequencing platforms for metagenomic sequencing and identification of pathogens in blood

Table 2 Cross-platform comparison of read mapping to Influenza A H1N1

Platform	#/Replicates	Reads mapped (low)	Reads mapped per 100,000^a	Reads mapped (med)	Reads mapped in pairs^a	Fraction of reference covered^a
PGM 1	3	11/20/ND	.61/.80/ND	5/16/ND	NA	.07/.15/ND
PGM 2	3	21/19/18	.87/1.3/.86	14/11/10	NA	.12/.09/.14
Roche	2	1/0	.17/0	0/0	NA	.03/-
MiSeq 1	3	308/322/323	.82/.90/1.0	269/311/318	66/72/82	.79/.95/.80
MiSeq 2	3	811/942/912	2.2/2.4/2.2	741/934/914	296/216/248	.95/.95/.97

ISSN: 1471-2164