Validation of CNVR410 by qPCR in another 16 chickens. X-axis represents all 16 samples and Y-axis represents normalized ratios (NR) estimated by qPCR. NR around 2 indicates normal status (2 copies), NR around 0 or 1 indicates loss status (0 copies or 1 copy), and NR around 3 or more indicates gain status (3 or more copies). (A) qPCR results confirmed by primer410_2. (B) qPCR results confirmed by primer410_3.