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Figure 8 | BMC Genomics

Figure 8

From: A deep transcriptomic analysis of pod development in the vanilla orchid (Vanilla planifolia)

Figure 8

Validation of lignin-related gene expression in the early stage seeds and stems. qRT-PCR was performed to verify the expression patterns of PAL, 4CL, CCR, CAD, C4H and CCoAOMT in the light and dark pod tissues, seeds at 6, 8 and 10 weeks and in stems. Expression values were normalized to expression levels of actin, and the expression value in 6 W dark was set to 1. The results are the means of duplicate measurements of two biological replicates, and p-values for difference in expression between 8 week seed tissue and 6 week dark pod tissues were less than 0.01 (Student’s t-test) for all transcripts tested.

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