Transcriptional responses of PBMC in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs

Table 2 Primer used to verify microarray experiments by qPCR

Gene symbol	Sequence 5' - 3' For	Sequence 5' - 3' Rev	Size (bp)
CCR1	CATTCCAGAAGATTGGGACAA	TGGCTCCAGGCTCATAGTAGA	182
CD14	GAGGTGGCAGAGTTCAAAGAG	CATGGTCGATAAGGTCCTCAA	196
TLR2	TAAGTTGAAGACGCTCCCAGA	ACAGGAAGTCACAGGAGCAGA	167
TLR4	CTCTGCCTTCACTACAGAGA	TTGAGTCGTCTCCAGAAGAT	323
TREM1	GGGAGAGACCCTGAATGTGA	ATCTTCCCCACCTGGACTTTA	156
HPRT1*	GTGATAGATCCATTCCTATGACT	TGAGAGATCATCTCCACCAATTA	104
PPIA*	GATTTATGTGCCAGGGTGGT	CTTGGCAGTGCAAATGAAAA	179
RPL32*	AGCCCAAGATCGTCAAAAAG	TGTTGCTCCCATAACCAATG	165

CCR1 – chemokine (C-C motif) receptor 1; CD14 – CD14 molecule; TLR2 – toll-like receptor 2; TLR4 – toll-like receptor 4; TREM1 – triggering receptor expressed on myeloid cells 1 precursor; HPRT1 – hypoxanthine phosphoribosyltransferase 1; PPIA – peptidylprolylisomerase A (cyclophilin A); RPL32 – ribosomal protein 32; * reference gene.

ISSN: 1471-2164