Expression patterns of Pfam domains among the most highly expressed transcripts. (A) Principal component analysis (PCA) of the abundance of Pfam domains. Each point in the PCA plot corresponds to a cDNA library. Ao(Mh) denotes A. oligospora and M. hapla; Ao(Hs), A. oligospora and H. schachtii; Ad(Mh), A. dactyloides and M. hapla; Ad(Hs), A. dactyloides and H. schachtii; and Mc(Hs), M. cionopagum and H. schachtii. The PCA was performed on the read counts of 700 Pfam domains (log2 transformed (counts + 1)). (B) Venn diagram of highly expressed Pfam domains. Shown is the distribution of the in total 700 unique Pfam domains that were found among the top 500 most expressed transcripts in A. oligospora (Ao), A. dactyloides (Ad) and M. cionopagum (Mc). ‘Ao’ contains all domains that were found among the 500 most expressed transcript in one or in both of the Ao(Mh) and Ao(Hs) libraries. ‘Ad’ contains all domains that were found among the 500 most expressed transcripts in one or in both of the Ad(Mh) and Ad(Hs) libraries. ‘Mc’ contains all domains that were found among the 500 most expressed transcript in Mc(Hs).