Interspecific and host-related gene expression patterns in nematode-trapping fungi

BMC Genomics

Table 5 Differentially expressed genes encoding secreted proteins in A. oligospora during infection of M. hapla or H. schachtii ^a

UniProt ID	Description^b	Pfam	Length^c	Fold change
Upregulated in M. hapla
G1X4P0	Histidine acid phosphatase	His_Phos_2	476	9.1
G1XTC9	Patched sphingolipid transporter	Patched	1 292	9.1
G1XF88	Uncharacterized protein	-	251^e	7.7
G1XGH3	IgE-binding protein	-	195	7.4
G1XHE8^d	-	-	196^e	6.9
G1X1U4	Glucosidase 2 subunit beta	-	553	6.3
G1X8R3	GPI anchored cell-wall protein	But2	328	6.2
G1XCM7	Beta-galactosidase	Glyco_hydro_35, BetaGal_ dom2, BetaGal_dom3, BetaGal_dom4_5	982	5.7
G1XET4	Peptidase S41	Peptidase_S41	702	5.7
G1XGI5	Uncharacterized protein	-	107^e	5.3
G1XPV3	Uncharacterized protein	-	173^e	5.3
G1XJP0	Uncharacterized protein	-	606	5.1
G1XLE5	Aminopeptidase Y	PA, Peptidase_M28	503	5.1
Upregulated in H. schachtii
G1X7Q6^d	-	-	300	15.8
G1XQA9	Uncharacterized protein	-	707	15.8
G1XU57	ABC transporter	ABC_tran, ABC2_membrane	1 047	15.8
G1X110^d	-	-	151^e	11.4
G1XR64	Uncharacterized protein	DUF3129	656	10.5
G1XF27	WSC-domain-containing protein	WSC	625	9.6
G1XEV7	Chitinase	Glyco_hydro_18, LysM	1 232	8.8
G1XM84	Tyrosinase	Tyrosinase	387	8.8
G1XC55	Uncharacterized protein	DUF3129	454	6.4

^aGenes encoding proteins with a predicted secretion signal that were upregulated at least 5-fold in the sample A. oligospora infecting M. hapla (Ao(Mh)) compared to the sample A. oligospora infecting H. schachtii (Ao(Hs)) and in Ao(Hs) compared to Ao(Mh). Genes that were expressed (≥1 read) in both libraries and that had ≥5 read in any of the libraries were included in the analysis, in total 4 138 genes.
^bNon-annotated genes of A. oligospora were further characterized by searches through the UniProt database [47] using the BLASTP algorithm [25] with an E-value threshold of 1e-10. Fungal sequences were chosen among the top hits.
^cThe length of the protein sequence in aa.
^dOrphan, protein that lacks known homologs and does not contain any Pfam domains.
^eSmall secreted protein (SSP) with unknown function. SSPs were defined as secreted proteins with a length of less than 300 aa.

ISSN: 1471-2164