Figure 6

Transient assays of Phytophthora RXLR, NLP and elicitin effectors in N. benthamiana . The P. cactorum elicitin PcELL1 (A), NLP PcNLP1 (B), four RXLRs (PcRXLR6, PcRXLR7, PcRXLR13 and PcRXLR27) [(C)-(F) in order], P. capsici RXLR PcapRXLR7 (G) and the alleles of PcRXLR6 and PcRXLR7 (H) triggered plant cell death. The plant leaves were infiltrated with A. tumefaciens (strain GV3101) cells to express each of these effectors. Agro-infiltration sites in each N. benthamiana leaf expressing INF1 (top left) were the positive controls. The other sites in leaves expressing GFP only, or infiltrated by 10 mM MgCl₂ buffer only served as negative controls. PcapRXLR7 is the ortholog gene of PcRXLR7 in P. capsici. The alleles of PcRXLR6 in the isolates PH-1-14-04 and PH-1-8-03 are labeled as PcRXLR6 (PH-1-14-04) and PcRXLR6 (PH-1-8-03), respectively while the allele of PcRXLR7 in PC7 as PcRXLR7 (PC7). Photographs were taken 5 [(A)-(C), (E)-(F) and (H)] or 7 [(D), (G)] d after infiltration. The circles indicated the agroinfiltration areas.