Figure 4

Predicted protein interaction networks involved in axonal injury. Prediction of upstream regulators was performed using IPA. The predicted upstream regulators (white nodes) were ATF4, IFNG, TP53, P38MAPK, ALDH2, and ACOX1. The targets of these upstream regulators are also displayed to illustrate the alteration in the interaction networks after ONC. Solid lines represent a direct interaction between two genes, whereas dotted lines represent an indirect relationship. The length of a line reflects the strength of reported evidence supporting the node-to-node relationship. The shapes of the nodes represent the different known biological roles of each of these molecules, as shown in the lower right inset. Red indicates up-regulated genes, green indicates down-regulated genes, and white indicates genes that were not annotated in these CAGE results, but that formed part of the network. *:targets that were duplicated in the dataset.